Two sheets in MS Excel file: (i) zebrafish_probes_PM, and (ii) mouse_probes_PM. PM, perfect match probes. Each sheet has two columns: first column is Agilent Probe ID and second column is DNA sequence
50 ng and/or approximately 16,000 copies of DNA was added to each real-time PCR mixture. The threshold was set at 10% of the average fluorescence signal of the reference assay at cycle 45.
