Transcriptomics of siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues

RNA was isolated from siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues using the TRIzol (Invitrogen) reagent by following the company manual. Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer's protocol.The libraries were sequenced using HiSeq3000 (Illumina) or HiSeq2500 in paired-read mode, creating reads with a length of 101 or 125 bp. Sequencing chemistry v2 or v4 (Illumina) was used. Examination of gene expressive levels in siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues采用TRIzol（Invitrogen）试剂并依照产品手册操作流程，从siCTRL、siNSUN2处理及ALYREF-RIP的HeLa细胞与多种小鼠组织中分离RNA。随后取约2.5 μg总RNA，依照厂商提供的实验方案，使用TruSeq RNA样本制备试剂盒v2（Illumina，美国加利福尼亚州圣地亚哥）完成文库构建。采用HiSeq3000（Illumina）或HiSeq2500测序平台，以双端测序模式对文库进行测序，得到长度为101 bp或125 bp的测序读段，测序使用v2或v4版本的测序化学试剂（Illumina）。本研究将对siCTRL、siNSUN2处理及ALYREF-RIP的HeLa细胞与多种小鼠组织的基因表达水平开展检测。