NIH 3T3 cells were treated with or without 30 nM ShhN and the cholesterol in cell extracts was quantified by gas chromatography in three independent experiments.
Unesterified (free) and esterified cholesterol was analyzed by HPLC and normalized to cell protein. Values are mean (± S.D.) of six separate cultures. Values in brackets give the percentage of esterif
The first column shows the values obtained by cholesterol quantification in the fluorimeter, the second column enumerates the values of total protein amounts obtained by Bradford method and the third
PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalizatio