2189N and 2190N Ruminant cattle myeloid cell subsets

Description Expression profiling by high throughput sequencing of ruminant (cattle and sheep) myeloid cells: 36 samples were profiled in total; derived from four biological replicates (i.e. isolated from four cattle or sheep). The myeloid subsets (of 4 individuals) were all derived from peripheral blood mononuclear cell preparations as follows: Sheep: (a) monocytes - purified by CD14+ MACS separation; (b) monocyte derived dendritic cells - cultured CD14+ monocytes with ovine IL-4 & GMCSF; (c) monocyte derived macrophages (MDM) - cultured adherent cells; (d) Chlamydia infected MDM. Cattle: (a) CD14+ monocytes - purified by CD14+ MACS separation; (b) CD16hi++ monocytes - purified by CD16+ MACS separation, followed by flow cytometry to obtain the CD16hi++ monocytes (c) monocyte derived dendritic cells - cultured CD14+ monocytes with bovine IL-4 & GMCSF; (d) monocyte derived macrophages (MDM) - cultured adherent cells; (e) Chlamydia infected MDM.