Cell-sheet shape transformation by internally-driven, oriented forces

During morphogenesis, cells collectively execute directional forces that drive the programmed folding and growth of the layers, forming tissues and organs. The ability to recapitulate aspects of these processes in vitro will constitute a significant leap forward in the field of tissue engineering. Free-standing, self-organizing, cell-laden matrices are fabricated using a sequential deposition approach that uses liquid crystal-templated hydrogel fibers to direct cell arrangements. The orientation of hydrogel fibers is controlled using flow or boundary cues, while their microstructures are controlled by depletion interaction and probed by scattering and microscopy. These fibers effectively direct cells embedded in a collagen matrix, creating multilayer structures through contact guidance and by leveraging steric interactions amongst the cells. In uniformly aligned cell matrices, oriented cells exert traction forces that can induce preferential contraction of the matrix. Simultaneously, th..., , # Cell-Sheet Shape Transformation by Internally-Driven, Oriented Forces

Dataset DOI: [10.5061/dryad.8gtht770q](10.5061/dryad.8gtht770q)

## Description of the data and file structure

Title of Dataset: Cell-Sheet Shape Transformation by Internally-Driven, Oriented Forces

This dataset contains the data presented in the main text figures and supplemental information of \"Cell-Sheet Shape Transformation by Internally-Driven, Oriented Forces\", by authors Junrou Huang, Juan Chen, and Yimin Luo in Advanced Material. (Journal citation: J. Huang, J. Chen, Y. Luo, Adv. Mater., 2025, 2416624. [http://doi.org/10.1002/adma.202416624](http://doi.org/10.1002/adma.202416624)).

Descriptions of the data and file structure:

Data are all provided in comma separated value form (CSV). File names refer to the figures numbers of the manuscript. Processing scripts and image analyzed are also uploaded. If the letter S is provided before the number, the figure can be found in the Supporting Information.

**Ma...,

在形态发生过程中，细胞集体施加定向作用力，驱动各层组织按程序折叠与生长，最终形成组织与器官。若能在体外重现该过程的部分关键环节，将为组织工程领域带来重大突破。本研究采用分步沉积法制备自立式、自组织的载细胞基质：以液晶模板化（liquid crystal-templated）水凝胶纤维引导细胞排布。水凝胶纤维的取向可通过流场或边界信号调控，其微观结构则由耗竭相互作用（depletion interaction）控制，并通过散射法与显微镜技术进行表征。此类纤维可有效引导嵌入胶原蛋白基质中的细胞，通过接触引导（contact guidance）以及利用细胞间的空间位阻相互作用（steric interactions）构建多层结构。在均匀取向的细胞基质中，定向排列的细胞会产生牵引力（traction forces），进而诱导基质发生优先收缩。与此同时，……，# 基于内驱定向力的细胞片层形态转变

数据集DOI：[10.5061/dryad.8gtht770q](10.5061/dryad.8gtht770q)

## 数据与文件结构说明
### 数据集标题：基于内驱定向力的细胞片层形态转变（Cell-Sheet Shape Transformation by Internally-Driven, Oriented Forces）
本数据集包含发表于《Advanced Materials》（《先进材料》）的论文《基于内驱定向力的细胞片层形态转变》（Cell-Sheet Shape Transformation by Internally-Driven, Oriented Forces）正文中的图表数据及补充材料数据，该论文作者为Junrou Huang、Juan Chen与Yimin Luo，期刊引用信息为：J. Huang, J. Chen, Y. Luo, Adv. Mater., 2025, 2416624. [http://doi.org/10.1002/adma.202416624](http://doi.org/10.1002/adma.202416624)。

数据与文件结构说明：
所有数据均以逗号分隔值（Comma Separated Value, CSV）格式提供。文件名对应论文中的图表编号。本次上传还包含处理脚本与图像分析文件。若文件名中数字前带有字母S，则该图表来自补充材料。
**……**