LC-MS/MS Datasets for PICS-based Active Site Specificity Profiling of Prolyl Endopeptidases Celiacase and Neprosin Variant XGR3 using E. coli-derived Peptide Libraries and Gliadin Substrates.

1. Celiacase (Neprosin Variant N2) - PICS Data (Tables 1A - 3B). Substrate: Trypsin-derived E. coli peptide library Table 1A: All Peptide-Spectrum Matches (PSMs) identified by LC-MS/MS. Table 1B: Protease cleavage specificity analysis using a dimethylation-based PICS workflow. Associated File: 20220923_CRJL_M9H11_PICS_EColi_Tryps_Neprosin_N2_5ug_155.raw Substrate: GluC-derived E. coli peptide library Table 2A: All Peptide-Spectrum Matches (PSMs) identified by LC-MS/MS. Table 2B: Protease cleavage specificity analysis using a dimethylation-based PICS workflow. Associated File: 20220923_CRJL_M9H11_PICS_EColi_GluC_Neprosin_N2_5ug_162.raw Substrate: Sigma Gliadin G3375 Table 3A: All Peptide-Spectrum Matches (PSMs) identified by LC-MS/MS. Table 3B: Protease cleavage specificity analysis using a modified PICS workflow. Associated File: 20220927_CRJL_M9H11_Gliadin_Neprosin_N2_175.raw 2. XGR3 (Neprosin Variant N3) - PICS Data (Tables 4A - 6B). Substrate: Trypsin-derived E. coli peptide library Table 4A: All Peptide-Spectrum Matches (PSMs) identified by LC-MS/MS. Table 4B: Protease cleavage specificity analysis using a dimethylation-based PICS workflow. Associated File: 20220923_CRJL_M9H11_PICS_EColi_Tryps_Neprosin_N3_5ug_156.raw Substrate: GluC-derived E. coli peptide library Table 5A: All Peptide-Spectrum Matches (PSMs) identified by LC-MS/MS. Table 5B: Protease cleavage specificity analysis using a dimethylation-based PICS workflow. Associated File: 20220923_CRJL_M9H11_PICS_EColi_GluC_Neprosin_N3_5ug_163.raw Substrate: Sigma Gliadin G3375 Table 6A: All Peptide-Spectrum Matches (PSMs) identified by LC-MS/MS. Table 6B: Protease cleavage specificity analysis using a modified PICS workflow. Associated File: 20220922_CRJL_M9H11_Gliadin_Neprosin_N3_150.raw