Figure 2. 14-3-3 proteins do not affect the total expression level of Nav1.5 and cell surface density.

Figure 2. 14-3-3 proteins do not affect the total expression level of Nav1.5 and cell surface density. Representative immunoblots of the total lysate and cell surface fraction of tsA201 stably expressing Nav1.5 after 48 hours of transient overexpression of: (a) HA-tagged 14-3-3 proteins; (c) YFP-tagged difopein and its mutant. Endogenous Na+/K+-ATPase was used as a positive control of cell surface fraction, while α-actin was used as a negative control. (b and d) Intensities of Nav1.5 in total protein lysate and at the cell surface were normalized to Na+/K+-ATPase and α-actin and to the control condition ("+ empty vector"). Data are presented as mean ± SEM from three-five biological replicates. Individual p-values, calculated with one-sample two-tailed t-test with hypothetical mean value=1, are indicated in each panel.