Around 20 NSN- or SN-GVOs per replica were collected and processed to obtain hairpin-bisulfite amplicons for major satellites and IAP_LTR1a. Briefly, 100 ng salmon sperm DNA was added as carrier DNA a
Monocytic THP1 cells were treated with PMA to differentiate them into macrophages. These macrophages were infected with H37Rv at an MOI of 10, and genomic DNA was isolated 48 hours after infection. Me
To identify parent-of-origin biased DNA methylation, we performed high-resolution bisulfite sequencing of chromosome 16 on peripheral blood and cultured skin fibroblasts from individuals with maternal
