Comparison of microarray results and qPCR validation data for neuronally differentiated SK-N-SH cells infected with either MECP2e1 or e2.

F test was calculated for pairwise comparison of RT-PCR results for MECP2e1 or e2 versus eGFP infection. Comparison with published data from knockout (null) and transgenic mouse gene expression microarray data (Chahrour et al, 2008 [33]) is shown. Overlap with several other gene expression/transcriptome analysis studies (Nectoux et al, 2010 [32]; Yakabe et al, 2008 [31]) is also commented on (see footnotes). 1Chahrour et al, 2008. %no expression detectable by qRT-PCR in control cells (eGFP infected), thus no fold-change calculation possible. *too low to measure. NA = not applicable (i.e. unable to calculate p-value). NC = no call (i.e. microarray didn’t show any fold change in expression). &from quantitative RT-PCR data. #Also identified as up-regulated in transcriptome analysis of Rett patients; Nectoux et al, 2010 [32]. $Also identified as up-regulated after siRNA knockdown of MECP2 in human derived cell-lines; Yakabe et al, 2008 [31].