microRNAs profiles of contrast-induced acute kidney injury rats

Contrast-induced acute kidney injury (CI-AKI) is typically defined by an increase in serum creatinine (SCr) after intravascular administration of contrast medium. Since creatinine is an unreliable indicator for acute changes in kidney function, an early biomarkers for CI-AKI diagnosis is important for initiating therapy.We assessed the hypothesis that circulating microRNAs (miRNAs) could be served as potential biomarkers to early detect CI-AKI.The rat model of acute kidney injury was developed as we previously described. We first detect miRNA profile of plasma and kidney tissue using Agilent microarray platform. 3 miRNA species with > 1.5-fold increase in plasma samples of CI-AKI rats, including miRNA-30a, miRNA-30e and miRNA-188, were selected as candidate miRNAs of potential biomarkers.造影剂诱导的急性肾损伤（Contrast-induced acute kidney injury, CI-AKI）通常被定义为血管内给予造影剂后血清肌酐（serum creatinine, SCr）水平升高。由于肌酐并非反映肾功能急性变化的可靠指标，因此开发可用于CI-AKI早期诊断的生物标志物对及时启动治疗至关重要。本研究验证了"循环微RNA（circulating microRNAs, miRNAs）可作为潜在生物标志物实现CI-AKI早期检测"这一假说。我们按照此前报道的方法构建了急性肾损伤大鼠模型，首先采用安捷伦微阵列平台（Agilent microarray platform）检测大鼠血浆与肾组织的miRNA表达谱。在CI-AKI模型大鼠的血浆样本中，筛选出表达上调超过1.5倍的3种miRNA，即miRNA-30a、miRNA-30e与miRNA-188，将其作为潜在生物标志物的候选miRNA。