Whole genome sequencing for Mack Super Snow leopard gecko mapping

We crossed two Mack Snow female leopard gecko (+/-) and a Mack Super Snow male (-/-) to obtain homozygous and heterozygous offspring for the Mack Super Snow locus. We sequenced whole-genome libraries of the parental DNA samples, pooling the females, and of two DNA pools from homozygous and heterozygous offspring in order to retrieve a mutation concordant with the Mack Super Snow genotype.