mRNA profiles of hematopoietic stem or progenitor cells in bone marrow after 5-FU administration

Purpose: The goals of this study are to elucidate the underlying mechanism for the regulation of HSC divisions. Methods: Before or after wild type (WT) mice were treated with 5-fluorouracil (5-FU), 100 cells of HSCs were sorted. Moreover, after HSCs were cultured for 1day, 100 cells of phenotypic HSCs were sorted. These cells were subjected to mRNA sequence using Next-seq. The sequence reads that passed quality filters were analyzed by CLC genomic workbench. Overall design: After 6 or 10 days from 5-FU administration, HSCs (CD150+ CD48- EPCR+ Lineage-) or HPCs (CD150+ CD48+ EPCR+ Lineage-) were sorted were sorted, and subsequently mRNA profiles were generated by deep sequencing using Next-seq. After culturing HSCs (CD150+ CD48- EPCR+ Lineage-) derived from untreated mice for 1 day, mRNA profiles were generated by deep sequencing using Next-seq .