METHODS AND COMPOSITIONS FOR IMPROVING ENGINEERED MICROBES

The present disclosure provides a bacterial composition， comprising: at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system， wherein the bacterial strain comprises a modification in or one or more genes selected from the group consisting of bcsll， bcslll， yjbE， fhaB， pehA， glgA， otsB， treZ， and cysZ. The present disclosure further provides a bacterial composition and method for increasing the colonization of a plant growth promoting bacterial strain on a plant， wherein the plant growth promoting bacterial strain has been remodeled to increase colonization of said plant， In a further aspect， the present disclosure provides methods of increasing nitrogen or nitrogen fixation available to a plant. The present disclosure provides a bacterial composition， comprising: at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system， wherein the bacterial strain comprises a modification in or one or more genes selected from the group consisting of bcsll， bcslll， yjbE， fhaB， pehA， glgA， otsB， treZ， and cysZ. The present disclosure further provides a bacterial composition and method for increasing the colonization of a plant growth promoting bacterial strain on a plant， wherein the plant growth promoting bacterial strain has been remodeled to increase colonization of said plant， In a further aspect， the present disclosure provides methods of increasing nitrogen or nitrogen fixation available to a plant.