Protein kinase C regulates the expression of cell wall-related genes in RlmA-dependent and independent manners in <i>Aspergillus nidulans</i>
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A protein kinase C of <i>Aspergillus nidulans</i>, PkcA, is required for cell wall integrity (CWI) and is considered a major component of the regulating pathway. To investigate whether PkcA regulates the transcription of cell wall-related genes, we constructed strains expressing <i>pkcA</i>(R429A) that encodes an activated form of PkcA. The mRNA levels of most chitin synthase genes and an α-glucan synthase gene, <i>agsB</i>, were increased when <i>pkcA</i>(R429A) expression was induced. These mRNA increases were not observed or were only partially observed, in a deletion mutant of <i>rlmA</i>, an ortholog of <i>RLM1</i> that encodes a transcription factor in the CWI pathway in <i>Saccharomyces cerevisiae</i>. In addition, in a <i>pkcA</i> temperature-sensitive mutant under heat stress, the mRNA levels of some chitin synthase genes and <i>agsB</i> did not increase. These results suggest that PkcA is involved in CWI maintenance through the transcriptional regulation of cell wall-related genes.
构巢曲霉（Aspergillus nidulans）的蛋白激酶C（Protein Kinase C）PkcA是细胞壁完整性（cell wall integrity, CWI）维持所必需的，且被视作该调控通路的核心组分。为探究PkcA是否调控细胞壁相关基因的转录，我们构建了表达pkcA(R429A)的菌株，该基因编码PkcA的激活型变体。当诱导pkcA(R429A)表达时，多数几丁质合酶基因（chitin synthase gene）与α-葡聚糖合酶基因（α-glucan synthase gene）agsB的mRNA水平均出现上调。在rlmA缺失突变体——该基因是酿酒酵母（Saccharomyces cerevisiae）CWI通路中转录因子（transcription factor）RLM1的同源基因——中，上述mRNA水平上调的现象未被观测到，或仅部分出现。此外，在热胁迫（heat stress）条件下的pkcA温度敏感型突变体中，部分几丁质合酶基因及agsB的mRNA水平未出现上调。上述结果表明，PkcA可通过转录调控细胞壁相关基因，参与细胞壁完整性的维持。
