The results obtained by QRT-PCR were expressed as ΔCT between the Ct obtained for the SR and β-Actin. The higher is the ΔCT, the lower mRNA expression level is (n = 4; mean ± SEM).
To parameterize our computational model of E. coli, we measured gene expression in key conditions. Overall design: For each condition, we measured 3 biological replicates (each replicate cultured on a