Transcriptional profiling of striatum in METH self-administered rats [mRNA]

This study aimed to identify changes in novel miRNAs and their target genes during METH self-administration and investigate their roles in METH-induced locomotion. RNA sequencing analysis revealed that mir-183-5p was upregulated in the striatum of METH self-administered rats, and target gene prediction revealed that the glucocorticoid receptor (GR) gene, Nr3c1, was a potential target gene for mir-183-5p. We confirmed that single and repeated METH administrations increased METH-induced locomotion and plasma corticosterone levels in rats.本研究旨在明确甲基苯丙胺（METH）自身给药过程中新型微小RNA（miRNAs）及其靶基因的表达变化，并探究其在甲基苯丙胺诱导的运动活动中的作用。RNA测序（RNA sequencing）分析显示，甲基苯丙胺自身给药大鼠的纹状体中mir-183-5p表达上调；靶基因预测结果表明，糖皮质激素受体（GR）基因Nr3c1是mir-183-5p的潜在靶基因。本研究证实，单次及重复给予甲基苯丙胺可增强大鼠的甲基苯丙胺诱导运动能力，并升高其血浆皮质酮水平。