Systems pharmacology demonstrates broad-spectrum efficacy in animal models of retinopathies [scRNA-seq: Rd10]

To determine the gene expression changes in the retina of 2-month-old Rd10 mice reared in darkness throughout their lifespan, we collected retina samples from Rd10 and WT (C57BL/6J mice) mice, generated single-cell suspensions, and performed scRNA-Seq (10X Genomics). One group of Rd10 mice was treated for 1 month via dietary administration of tamsulosin, metoprolol and bromocriptine drug cocktail. Vehicle- and TMB-treated Rd10 mice: One retina from four female Rd10 mice were pooled to prepare single-cell suspensions at a final concentration of 1,000 cells/l. WT mice: One retina from two female and two male C57BL/6J mice were pooled to prepare single-cell suspensions at a final concentration of 1,000 cells/l. For each group, freshly dissociated cells (~16,500) were loaded into a 10x Genomics Chromium Single Cell system using v3 chemistry following the manufacturer's instructions.为探究终生饲养于黑暗环境中的2月龄Rd10小鼠视网膜内的基因表达变化，我们收集了Rd10小鼠与野生型（WT，C57BL/6J品系小鼠）的视网膜样本，制备单细胞悬液并开展基于10X Genomics平台的单细胞RNA测序（scRNA-Seq）。其中一组Rd10小鼠通过饮食给药方式，接受为期1个月的坦索罗辛、美托洛尔与溴隐亭联合药物鸡尾酒疗法。 针对赋形剂处理组与TMB处理组的Rd10小鼠：将4只雌性Rd10小鼠的单侧视网膜组织混合，制备终浓度为1000个细胞/升的单细胞悬液；野生型小鼠组：将2只雌性与2只雄性C57BL/6J小鼠的单侧视网膜组织混合，制备终浓度为1000个细胞/升的单细胞悬液。 针对每个实验组，我们依照制造商的操作说明，采用v3化学试剂盒，将约16500个新鲜解离的细胞上样至10x Genomics Chromium单细胞系统中。