Single-cell RNA-seq analysis of WT and Furin KO TECs

To assess the effect of Furin ablation on TEC development and heterogeneity, we detected the transcriptional profiles of 2-week-old WT and Furin KO TECs using single-cell RNA-seq. Employing an unsupervised graph-based clustering strategy, 20 clusters of TECs and 5 clusters of non-TEC contaminants were identified. After removing contaminants, 4301 WT TECs and 4086 Furin KO TECs were obtained. UMAP visualization revealed that the proportion of mTECs decreased significantly and the proportion of cTECs increased significantly in TEC-specific Furin knockout mice in comparison with WT mice. Further analysis showed that Furin ablation did not affect the proportion of mTEC I, II and III subsets, but reduced the proportion of mTEC IV subsets markedly. Compare the transcriptional profiles of 2-week-old WT and Furin KO TECs using single-cell RNA-seq为探究弗林酶（Furin）敲除对胸腺上皮细胞（TEC）发育与异质性的影响，本研究采用单细胞RNA测序技术，对2周龄野生型（WT）与弗林酶敲除（Furin KO）胸腺上皮细胞的转录表达谱进行检测。通过无监督图基聚类策略，本研究共鉴定出20个胸腺上皮细胞簇与5个非胸腺上皮细胞污染簇。剔除污染簇后，最终获得4301个野生型胸腺上皮细胞与4086个弗林酶敲除胸腺上皮细胞。均匀流形近似与投影（UMAP）可视化结果显示，与野生型小鼠相比，胸腺上皮细胞特异性弗林酶敲除小鼠的髓系胸腺上皮细胞（mTEC）占比显著降低，皮质胸腺上皮细胞（cTEC）占比则显著升高。进一步分析表明，弗林酶敲除未对mTEC I、II、III亚群的占比产生影响，但可显著降低mTEC IV亚群的占比。本研究采用单细胞RNA测序技术，对比了2周龄野生型与弗林酶敲除胸腺上皮细胞的转录表达谱。