T cell antigen receptor d (Tcrd) variable region exons are assembled by RAG-initiated V(D)J recombination. Here, we employ a high throughput method to map hundreds of thousands of RAG-initiated Tcrd D
Chronic lymphocytic leukemia (CLL) is an indolent non-Hodgkin lymphoma characterized by tumor B-cells that weakly express a B cell receptor (BCR). The great majority of CLL B-cells are unclass-switche
Antigen receptor gene recombination requires stochastic, monoallelic choice of a single variable gene in each lymphocyte progenitor. However, how this occurs remains unknown. Herein, we report that pr
RAG endonuclease initiates V(D)J recombination in progenitor (pro)-B cells. Upon binding a recombination center (RC)-based JH, RAG scans upstream chromatin via loop extrusion, potentially mediated by