Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1); on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1); on Col-0 (Col-0/Col-0). Seeds were collected from the grafted Col-0 scion af
To identify parent-of-origin biased DNA methylation, we performed high-resolution bisulfite sequencing of chromosome 16 on peripheral blood and cultured skin fibroblasts from individuals with maternal
Around 20 NSN- or SN-GVOs per replica were collected and processed to obtain hairpin-bisulfite amplicons for major satellites and IAP_LTR1a. Briefly, 100 ng salmon sperm DNA was added as carrier DNA a
Pseudomonas fluorescens FW300-N1B4 E.coli validation experiment for multiplex DNA Affinity Purification (DAP) sequencing as part of a new protocol development at the Joint Genome Institute (JGI). This