Open Array Mouse Inflammation Real-time quantitative PCR analysis of mouse ileal mucosa

Intrarectally DNBS-treated groups, to provoke colitis, were treated orally with the commensal CEC (CEC) or probiotic Nissle 1917 (Nissle) E. coli strains. Healthy control group was treated intrarectally and orally with PBS (PP). Sick control group was treated intrarectally with DNBS and orally with PBS (DP). Mice were sacrificed 24 days post first DNBS injection and the ileal gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays Mouse Inflammation Panel. qPCR gene expression profiling. Ileal mucosa from healthy control groups mice were used as control. Equal amount total RNA from each mouse was pooled prior to gene expression analysis.