GWAS combined with QTL mapping reveals the genetic loci of leaf morphological characters in Nicotiana tabacum

In this study, germplasm collection of cigar tobacco containing 185 accessions and an F2 population consisting of 240 individuals were created and used for whole-genome resequencing. GWAS combined with QTL mapping were subsequently conducted using the phenotyping data of leaf morphological characters in cigar tobacco. The genetic loci associated with leaf thickness from a co-localized genomic region were revealed