Impact of MET2 gene deletion on the growth kinetics of C. albicans SC5314 cells

The datasets present results on the impact of MET2 gene deletion, which encodes homoserine acetyltransferase, on the growth kinetics of C. albicans SC5314 cells in various media. Overnight cultures of C. albicans were washed twice with sterile phosphate-buffered saline (PBS). The cell suspensions were diluted to 2 × 10⁵ cells mL⁻¹. Aliquots of 100 µL were used to inoculate microtiter plate wells containing 100 µL of the appropriate medium. The microplates were incubated at 30 °C for 24 h, and cell density was measured spectrophotometrically (λ = 600 nm) using a TECAN Spark 10M microplate reader. Media compositions: YNB medium (with ammonium sulfate): YNB medium without amino acids, with ammonium sulfate, 2% (w/v) glucose, supplemented with 5 mM L-Met. YNB medium (with sodium glutamate): YNB medium without amino acids and without ammonium sulfate, containing sodium glutamate, 2% (w/v) glucose, supplemented with 5 mM L-Met. YEPG medium: 1% (w/v) yeast extract, 1% (w/v) peptone, and 2% (w/v) glucose. RPMI 1640 medium: a balanced mixture of amino acids, vitamins, inorganic salts, and 2% (w/v) glucose, buffered with 0.165 M MOPS (pH 7.0). The studies were financially supported from the project OPUS 20 financed by the National Science Centre (No. UMO-2020/39/B/NZ7/01519).