Genome-derived Microsatellite Markers in <i>Lippia origanoides</i> from Colombia

Polymorphic microsatellite sequences were mined from genome sequences of two <i>Lippia origanoides</i> wild specimens from disjunct locations. Twenty-three primer sequences flanking the candidate markers were designed, and their usefulness for population genetic analysis was assessed in plants sampled from four municipalities in Colombia. A final set of 10 primer pairs was useful for diversity analyses, which indicated the existence of three genetic lineages in Colombia. Cross-amplification in five related <i>Lippia</i> species was validated. The findings supported the efficiency of <i>in silico</i> approaches in providing novel genetic resources for research in <i>Lippia</i> species.研究从两株采自间断分布生境的野生牛至叶过江藤（*Lippia origanoides*）标本的基因组序列中挖掘得到多态性微卫星序列。设计了23条靶向候选分子标记侧翼区域的引物序列，并以哥伦比亚4个市镇采集的植株为材料，评估了这些引物在群体遗传分析中的适用性。最终筛选得到的10对引物可有效用于遗传多样性分析，分析结果显示哥伦比亚境内存在3个遗传谱系。同时验证了该引物组在5个近缘过江藤属（*Lippia*）物种中的跨物种扩增效果。本研究结果证实，硅基实验（in silico）方法可为过江藤属物种的相关研究提供新型遗传资源，证明了该类方法的应用有效性。