Leptin and fractalkine: Novel subcutaneous cytokines in burn injury.

Burn injury remains a major clinical challenge to both survival and to quality of life. Its progressive, aberrant inflammation underlies the lethal dysfunction of various organs and the pain it induces is excruciating and notoriously difficult to manage. While it is known that burn injury’s complex local and disseminating pathology is orchestrated from the burned tissue, few studies have sought to characterise the local signalling environment. An enhanced understanding of the local and acutely temporally-dynamic processes defining burn injury and its progression is required for the development of novel therapeutic interventions. Microdialysis was used as an interstitial sampling technique, conducted over three hours post-burn. Samples were analysed by metabolomics and a multiplex cytokine immunoassay. Next-Generation sequencing libraries of the burn and control microdialysis sites were prepared to measure transcriptional changes potentially underlying the interstitial profile characterising burn injury.烧伤始终是威胁患者生存与生活质量的重大临床难题。其进行性异常炎症会引发多器官致命性功能障碍，而由此产生的疼痛剧烈难忍且极难管控。尽管已知烧伤复杂的局部与播散性病理改变由烧伤组织介导调控，但目前鲜有研究旨在刻画其局部信号微环境的特征。要开发新型治疗干预手段，需加深对定义烧伤及其病程进展的局部急性时序动态变化过程的理解。本研究采用微透析（Microdialysis）作为间质采样技术，于烧伤后三小时内完成采样。通过代谢组学与多重细胞因子免疫测定对采集样本进行分析。构建烧伤组与对照组微透析位点的下一代测序（Next-Generation sequencing）文库，以检测可能介导烧伤特征性间质特征谱的转录组变化。