CRISPR interference (CRISPRi), based the fusion of enzymatically inactive Cas9 (dCas9) to the KRAB repressor domain, has emerged as a powerful platform to silence gene expression and decipher the logi
Silencing of a subset of germline genes is dependent upon DNA methylation (DNAme) post-implantation. However, these genes are generally hypomethylated in the ICM, implicating alternative silencing pat
A long form (tRNase ZL) of tRNA 3′ processing endoribonuclease (tRNase Z, or 3′ tRNase) can cleave any target RNA at any desired site under the direction of artificial small guide RNA (sgRNA) that mim
