Diagnosis of Three Different Pathogenic Microorganisms by Gas chromatography–mass spectrometry

Monocultures of E. coli, S. aureus and C. albicans sub-cultured aerobically at 37°C in 30 ml of two different types of broth medium Mueller Hinton broth (MB) and tryptic soy broth (TSB) in 100 ml sterilized glass bottles. The headspace of each them was extracted in both media and at three different periods of 2, 4 and 24 hours. A Solid Phase Microextraction (SPME) fiber holder containing a fiber coated with 50/30 µm (DVB/CAR/PDMS) was used for absorption of volatile compounds from headspace of pathogens then the fiber was injected to GC-MS analysis.