These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same . cells described in the Science paper (Direct visualization of horizontal gene transfer). The . cells wer
The creation of platinum replicas on an EM grid was carried out as follows: unroofed cells on EM grids were fixed in 2% paraformaldehyde for 20 minutes, then in 2% glutaraldehyde at 4 °C overnight. Af