Summary of the basal phosphorylation and inhibitor-induced dephosphorylation of kinase-inactive LRRK2.

Summary of the basal phosphorylation (Fig. 2A–C) and inhibitor-induced dephosphorylation of Ser910, Ser935, and Ser955 (Figure 3). Theoretical results of the kinase-inactive mutant according to the hypothesis schematized in the Figure 1B are also included (theoretical kinase-inactive). For basal phosphorylation, mutants showing 10∼50% phosphorylation at corresponding residues were marked with single plus sign (+). Likewise, those showing 50∼75% and 75∼100% phosphorylation were marked with double plus signs (++) and triple plus signs (+++), respectively. For inhibitor-induced dephosphorylation, mutants which remained 75∼100% phosphorylated after inhibitor treatment were marked with minus sign (−). Likewise, those remaining 50∼75%, 25∼50% and 0∼25% phosphorylated were marked with single plus sign (+), double plus signs (++) and triple plus signs (+++), respectively. *Note that S2032A LRRK2 retains the kinase activity based on our results (Figure 5).