Integrative genome-wide analysis in mouse mammary tumors in response to maternal long-term genistein (LT-GE) treatment

We tested global gene transcriptome changes by RNA-sequencing analysis and genome-wide methylation profiles by reduced representation bisulfite sequencing (RRBS) analysis in the offspring mammary tumors of C3(1)-SV40 Tag (SV40) transgenic mice to further identify key epigenetic-controlled genes in regulation of maternal GE diet-mediated early breast cancer prevention. Overall design: Total RNA and genomic DNA samples obtained from the offspring mammary tumors of SV40 transgenic mice with mothers fed either control or LT-GE diets (maternal GE dietary administration began in early life from 4 weeks of age in mothers until weaning) were subjected RNA-seq analysis and RRBS analysis.