Colibactin self resistance protein ClbS and ClbS like protein sequence data from the NORM hybrid assembly collection

Contains data about the colibactin self resistance protein ClbS and a putative ClbS like protein used in the study "Co-evolution between colibactin production and resistance is linked to clonal expansions in Escherichia coli". References Data Isolation, sequencing, and assembly are described in the following two studies: [1] Gladstone et al. "Emergence and dissemination of antimicrobial resistance in Escherichia coli causing bloodstream infections in Norway in 2002–17: a nationwide, longitudinal, microbial population genomic study". The Lancet Microbe (2021). doi: 10.1016/S2666-5247(21)00031-8. [2] Arredondo-Alonso et al. "Plasmid-driven strategies for clone success in Escherichia coli". Nature communications (2025). doi: 10.1038/s41467-025-57940-1. Sequencing data is available from the European Nucleotide Archive under accession numbers PRJEB45354 and PRJEB57633. Genome annotation Genome annotation was performed using bakta v1.10.4 (GitHub: oschwengers/bakta) against full database v5.1 dated 2024-01-19 (doi: 10.5281/zenodo.10522951): [3] Schwengers et al. "Bakta: rapid and standardized annotation of bacterial genomes via alignment-free sequence identification." Microbial genomics (2021). doi: 10.1099/mgen.0.000685. Annotation files are available in a separate Zenodo upload: doi: 10.5281/zenodo.17453317. Protein alignment Protein sequence similarity was computed with foldmason [4] against protein structure predictions extracted from AlphaFold [5]. [4] Gilchrist, C. L. M., Mirdita, M. & Steinegger, M. "Multiple protein structure alignment at scale with FoldMason." bioRxiv (2024) doi: 10.1101/2024.08.01.606130. [5] Jumper, J. et al. "Highly accurate protein structure prediction with AlphaFold." Nature (2021). doi: 10.1038/s41586-021-03819-2. Nucleotide similarity Nucleotide sequence similarity was confirmed by using kbo [6] to find sequence homologs to representative clbS and clbS-like genes identified by protein alignment. [6] Mäklin, T., Alanko, J. N., Biagi, E. & Puglisi, S. J. "Sequence alignment with k-bounded matching statistics." bioRxiv (2025) doi: 10.1101/2025.05.19.654936.