Linkage Mapping Reveals Non-Association of Green Revolution Genes (<i>Rht1</i> and <i>Rht2</i>) with Drought Tolerance in Wheat (<i>Triticum aestivum</i>) - Raw Data

The impact of green revolution genes on rice (<i>Oryza sativa</i> L.) and wheat (<i>Triticum aestivum</i> L.) productivity under rainfed environments has been debated over the past few decades. Here, we tried to assess the impact of two major green revolution genes (<i>Rht1 B1</i> or <i>Rht1</i> and <i>Rht1D1</i> or <i>Rht2</i>) on grain yield under drought stress. A total of four recombinant inbred line (RIL) populations were analyzed for this objective, including the following: PBW343 × Muu, PBW343 × Kingbird, PBW343 × Kenyaswara, and Jal 95.4.3 × Kachu/Kiritati/Kachu. These populations were segregated by either or both <i>Rht</i> gene alleles. Our results revealed an invariable non-association of tall/dwarf alleles of <i>Rht B1</i> and <i>Rht D1 </i>genes with grain yield under drought stress. Tightly linked sequence tags with <i>Rht</i> and <i>Vrn</i> genes were identified for future application in wheat breeding. A genetic linkage map of 1170 DArt-seq markers covering 2870 cM was constructed in the Jal 95.4.3 × Kachu/Kiritati/Kachu RIL population and quantitative trait loci (QTLs) for yield-related traits were identified. This study provides an insight for researchers involved in developing the next generation of climate-resilient wheat varieties that can cope well with rainfed/drought-prone environments.