Evolution of Potato spindle tuber viroid quasi-species during the course of disease in tomato plants. Evolution of Potato spindle tuber viroid quasi-species during the course of disease in tomato plants

In order to verify the production of viroid specific small RNAs (vd-sRNA) by viroids upon infecting plants, the tomato plants (Lycopersicum esculentum cv. Rutgers) were inoculated with Potato spindle tuber viroid (PSTVd) variants. After 21-days of post inoculation, total RNA was extracted and subjected for deep-sequencing using Illumina platform. Obtained data was analyzed for the presence of PSTVd specific small RNAs. Overall design: Examination of viroid quasi-species at different stages of disease in tomato plants.