Proliferation analysis by a dye dilution

To demonstrate a dye dilution assay can be used to estimate the doubling time of cell lines in both monoculture and coculture. Conclusion: We quantitatively analyzed the proliferation of cell lines by estimating the doubling time of cell lines either in monoculture or coculture using MDNs (mean division numbers) obtained by dye dilution assays. We listed a set of experimental criteria for an accurate measurement of doubling times with the proposed method. FACSVerse system includes an automated laser alignment and performance QC was carried out with BD FACSuit CS&T Research Beads. Performance QC for FACSAria system was carried out with BD Cytometer Setup and Tracking Beads.