Effect of BMP signaling levels on foregut specification during directed differentiation of BU3 NGPT human induced pluripotent stem cells

To investigate the dose dependent effects of BMP signaling on foregut organ domain specification, we treated hiPSC derived definitive endoderm with 0µM, 1µM or 2µM of BMP inhibitor DSM during days 3-6 of directed differentiation. We then preformed gene expression analysis of D6 samples between the 3 treatment groups using data generated from the RNA seq Overall design: Differential gene expression analysis of iPC derived foregut treated with differential concentrations of BMP inhibitor Dorsomorphin