Adult male fly hemolymph Lipidomics Data on Normal (ND) and High Sugar (HSD) for 14 days.

Frozen hemolymph samples from ND and HSD-fed flies were sent to the Northwest Metabolomics Research Center (NW-MRC) for targeted quantitative lipid profiling using the Sciex 5500 Lipidyzer as per methods established by NW-MRC¹²⁰. The materials used include LC-MS grade methanol, dichloromethane, and ammonium acetate, all sourced from Fisher Scientific (Pittsburgh, PA). HPLC grade 1-propanol was obtained from Sigma-Aldrich (Saint Louis, MO). Milli-Q water was produced using an in-house Ultrapure Water System by EMD Millipore (Billerica, MA). The Lipidyzer isotope-labeled internal standards mixture, which contained 54 isotopes from 13 different lipid classes, was acquired from Sciex (Framingham, MA). HCER and LCER were not detected in <i>Drosophila </i>hemolymph, so we report 11 different lipid classes.