<b>Figure 4. Activation of innate immune signaling induces HSV-1 reactivation from latent infection.</b>
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4A-E. Neurons latently infected with Stayput-GFP were treated with ligands for the DNA/RNA sensing pathways: <br>4A. Number of Us11-GFP expressing neurons at 2 days post treatment with; Mock (not treated), LY294002 (20μM) and DMXAA (50 μg/mL). <br>4B. Number of Us11-GFP expressing neurons at 2 days post treatment with; Mock (not treated), LY294002 (20μM) and ADU-S100 (10 μg/mL).<br>4C. Number of Us11-GFP expressing neurons at 2 days post treatment with; Mock (not treated), LY294002 (20μM) and Poly(dA-dT)/LyoVec (5 μg/mL). <br>4D. Number of Us11-GFP expressing neurons at 2 days post treatment with; Mock (not treated), LY294002 (20μM) and ISD (5μg/mL; D) <br>4E. Number of Us11-GFP expressing neurons at 2 days post treatment with; Mock (not treated), LY294002 (20μM), Poly(I:C) (HMW), or Poly(I:C) (LMW) (10 μg/mL).<br>4F. Number of Us11-GFP expressing neurons at 2 days post co-infection or Mock (not treated). Neurons latently infected with Stayput-GFP were co-infected with the HCoV-OC43 at an MOI of 3 PFU/cell.4G. Number of Us11-GFP expressing neurons at 2 days post treatment with; Mock (not treated), DMXAA (50 μg/mL) and DMXAA (50 μg/mL) + GNE-3511(4 μΜ). Neurons latently infected with Stayput-GFP were reactivated with DMXAA in the presence or absence of the DLK inhibitor GNE-3511 (4 μΜ). 4H. Relative expression of <i>ICP27</i> mRNA quantified by RT-qPCR at 18 hours post-reactivation (Phase I). Neurons latently infected with KOS-SPA and KOS-UL98 were treated with either LY294002 or DMXAA or LY294002, followed 2.5 hours later by DMXAA.
