Average net photons acquired from a single bead by microscopes of different types, plotted against standardized illumination dosage, for three different levels of out-of-focus background (), on a logarithmic scale
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In the = 484 (100 μM AlexaFluor 488 background solution) plot, ‘SS2 HA’ indicates spot scanning confocal #2 operated with the pinhole set to 1/2 Airy disk diameter.<b>Copyright information:</b>Taken from "Evaluating performance in three-dimensional fluorescence microscopy"Journal of Microscopy 2007;228(3):390-405.Published online Jan 2007PMCID:PMC2438600.© 2007 The Authors Journal compilation ©2007 The Royal Microscopical Society
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2016-01-11
