Expression of Arabidopsis WRI1 and DGAT1 during soybean embryo development alters oil and carbohydrate composition

Soybean (Glycine max [L.] Merr.) is the largest feedstock for protein and second largest source of vegetable oil in the world. In order to evaluate whether the expression of ATWRI1 and AtDGAT1 could direct carbon flow towards lipid accumulation, we generated three independent transformation events (WD-1, -2, and -3). The transgenic events were evaluated in three field trials (2018, 2019, and 2020) as well as greenhouse conditions. RNAseq analysis was performed in immature embryos of both transgenic events grown under greenhouse conditions. We discovered that Glycolysis and FA metabolism enzymes are enriched in WD differentially expressed genes during seed development. To check the response of the expression of Arabidopsis WRI1 and DGAT1 during soybean embryo development to alter oil and carbohydrate composition in seeds