Data files supporting the manuscript 'A Pomegranate Polyphenol Extract Suppresses the Microbial Production of Proatherogenic Trimethylamine (TMA) in an In Vitro Human Colon Model'

This dataset contains LC-MS/MS quantifications of methylamines from an in vitro colon model study investigating the effects of a polyphenol-rich pomegranate extract on microbial trimethylamine (TMA) production from L-carnitine and choline. Batch fermentations were conducted using 1% human fecal inoculum, supplemented with either L-carnitine or choline, and incubated under anaerobic conditions (pH 6.6–7.1, 37°C) for 24 or 48 hours. Concentrations of L-carnitine, choline, gamma-butyrobetaine, and trimethylamine (TMA) were quantified using LC-MS/MS with isotopically labeled internal standards. This dataset includes the processed data of quantified methylamine concentrations. These data support the manuscript: J. E. Haarhuis, P. Day-Walsh, E. Shehata, G. M. Savva, B. Peck, M. Philo, P. A. Kroon, A Pomegranate Polyphenol Extract Suppresses the Microbial Production of Proatherogenic Trimethylamine (TMA) in an In Vitro Human Colon Model. Mol. Nutr. Food Res. 2025, e70166. https://doi.org/10.1002/mnfr.70166