Impact of LCL161 on gene expression in human TAC T cells stimulated with antigen-coated microbeads

SMAC mimetics such as LCL161 are a novel class of anti-cancer drug that have been shown to induce costimulation in human T cells and augment anti-tumor capabilities. We investigated what impacts LCL161 has on gene expression in engineered anti-BCMA TAC T cells. TAC T cells were stimulated with BCMA-coated microbeads to deliver controlled antigenic signal alone. 12 samples were analyzed, containing 3 biological (donor) replicates and 2 time points. Samples stimulated containing DMSO were control and used for pairwise comparison against stimulations containing LCL161 drug.