Spatial transcriptomics of mouse spleens during malaria convalescence.

Naturally-acquired immunity to blood-stage malaria is associated with several effector CD4 + T subsets including germinal centre (GC) Tfh, Th1 and Tr1 cells. Here, we mapped the locations of effector CD4+ T cell subsets during post-Plasmodium convalescence using Slide-seqV2. Mice were infected with Plasmodium chabaudi chabaudi AS (PcAS) parasites and mice were administered with artesunate (intermittent artesunate therapy - IAT) at Day 7. At Day 30, whole spleens were recovered and fresh frozen to generate Slide-seqV2 datasets.