RNA-SEQ of wheat Jing411

Exposure to cold conditions is a major abiotic stress affecting crop growth and productivity. Elucidation of genetic mechanisms underlying response to low temperature is needed. To assess the relationship between the genes and the cold tolerance of wheat, transcriptomics analyses were performed to study changes in gene profiles of cultivar Jing 411 after cold acclimation and freezing treatment. The wheat cultivar Jing 411 was subjected to transcriptome sequencing. The RNA was sequenced on a HiSeq 2500 platform, and the generated data were analyzed using TopHat v2.0. Four treatment (cold acclimation:LTA, unacclimation:NTG, acclimation and freezing:LTAF and unacclimation and freezing: NTGF) and each treatment for three biological replicates