In vitro assessment of Bacillus subtilis DSM29784 secreted metabolites on gut chicken microbiota

In poultry, the probiotic Bacillus subtilis 29784 (Bs29784) sustains intestinal health, enhancing animal resilience and performance through the production of the bioactive metabolites hypoxanthine (HPX), Niacin (NA), and pantothenate (PTH). Here, using enterocyte in vitro models, we determine the functional link between these metabolites and the three pillars of intestinal resilience: immune response, intestinal barrier, and microbiota. To this end, we evaluated in vitro Bs29784 vegetative cells, spores, and metabolites capacity to modulate global immune regulators (using HT-29-NF-κB and HT-29-AP1 reporter cells), intestinal integrity (HT-29-MUC-2 reporter cells and Caco-2 cells), and cytokine production (Caco-2 cells). Finally, we simulated chickens’ intestinal fermentations to determine the effect of Bs29784 metabolites on the microbiota and their fermentation profile. Bs29784 vegetative cells reduce the inflammatory response more effectively than spores, indicating that their benefit..., All experiments were conducted according to the European Union Guidelines of Animal Care and legislation governing the ethical treatment of animals, and investigators were certified by the French government to conduct animal experiments. The Center for Expertise and Research in Nutrition facilities (Malicorne, France) are in accordance with the agreement no. C 03 159 4 of 6 November 2008, relative to experimentation on vertebrate living animals (European regulation 24/11/86 86/609 CEE; Ministerial decree of 19 April 1988). Twelve 28-days old broilers chickens were euthanized and their cecal and ileal contents were collected and pooled and immediately stored at -80°C. Cecal and ileal chicken microbial fermentation was performed in Hungate tubes in anaerobic buffer prepared according to Davies et al. (2000). Four technical replicates were performed by condition. The anaerobic buffer was composed of 5 solutions (A, B, C, D and E) prepared individually: solution A (per Liter: 5.7 g Na2HPO4,..., , # In vitro assessment of Bacillus subtilis DSM29784 secreted metabolites on gut chicken microbiota [https://doi.org/10.5061/dryad.1rn8pk138 ](https://doi.org/10.5061/dryad.1rn8pk138) In this study, we aimed to investigate the mechanisms by molecules released by Bacillus subtilis DSM29784 modulate chicken gut microbiota, focusing on hypoxanthine (HPX), niacin (NIA) and panthotenate (PTH). We conducted in vitro assay targeting the modulation of microbiota by mimicking ileal and cecal chicken compartment thanks to the inocula fermentation of 28 days-old broiler chickens. There are 4 repetitions by condition tested. ## Description of the data and file structure This README file describes the data package accompanying the above publication: * *vieco-saiz_et_al_2024_Sequences folder* present[s](javascript:) the reads in fastq.gz format * *vieco-saiz_et_al_2024_metadata.txt* identifies the sequences and the conditions tested ## Code/Software The primer and index were identified (100%...