Single-particle tracking (SPT) data of MET with a non-activating Fab ligand and the activating ligand InlB321 recorded in HeLa cells

Universal point accumulation for imaging in nanoscale topography (uPAINT) was applied to measure the dynamics of the MET receptor in living HeLa cells. For the resting receptor, an ATTO 647N-labeled, non-activating Fab antibody fragment was used. The ligand-bound state was probed using the InlB321 ligand site-specifically labeled with ATTO 647N which was fully functional. Imaging was performed in total internal reflection fluorescence (TIRF) mode using an N-STORM microscope (Nikon, Japan).