full_reference_return_model.rds

Seurat object with the integrated reference scRNA-Seq dataset of peripheral blood CD4 T lymphocytes. The integrated dataset consists of new samples (Chudakov lab, to be published), and published data (Stephenson, E., Reynolds, G., Botting, R.A. et al. Single-cell multi-omics analysis of the immune response in COVID-19. Nat Med 27, 904–916 (2021). https://doi.org/10.1038/s41591-021-01329-2). Cell barcodes in samples from Stephenson et al. are consistent with the original paper. Seurat metadata includes the most frequent TCR alpha and TCR beta clonotypes in cells. Metadata columns starting with "sortseq_" contain frequencies of the given clonotypes in repertoires of sorted Th subsets from Kasataskaya et al. (Sofya A Kasatskaya, et al. (2020) Functionally specialized human CD4+ T-cell subsets express physicochemically distinct TCRs eLife 9:e57063. https://doi.org/10.7554/eLife.57063)