Transcriptome analysis of Aofus3 in nematode-trapping fungus Arthrobotrys oligospora

Transcriptomic analysis revealed that differentially expressed genes in the absence of Aofus3 were involved in cell fusion, repair, and cellcommunication during trap formation. WT and ΔAofus3 strains grown on cellophane-overlaid PDA plates at 28°C for 5 days. Approximately 600 nematodes were then added to each plate for 0, 12, 24, and 36 h of induction, three replicates were used for each sample. After collecting the samples, RNA was extracted with the AxyPrep Multisource RNA Miniprep Kit (Axygen, Jiangsu, China), and then sent to Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China) to construct the transcriptome of WT and ΔAofus3 strains. The final cDNA library was sequenced on an Illumina Novaseq 6000 platform.