Summary of results of velocity experiment of HA-RBD in 10 mM Tris pH 8, 100 mM NaCl at 20°C.

aConcentration of peak in mg/mL in parenthesis. bSedimentation coefficient taken from the ordinate maximum of each peak in the best-fit c(s) distribution at 20°C with percentage protein amount in parenthesis. Sedimentation coefficient (s-value) is a measure of the size and shape of a protein in a solution with a specific density and viscosity at a specific temperature. cStandard sedimentation coefficient (s020,w -value) at zero concentration, in water at 20°C. dMolar mass values taken from the c(s) distribution that was transformed to the c(M) distribution. The theoretical mass of the monomer is given in parenthesis. eBest-fit weight-average frictional ratio values (f/f0)w taken from the c(s) distribution. The frictional ratios calculated with s020,w -values via the v-bar method (SEDNTERP) is in parenthesis.