Transgenic expression of human C19MC miRNAs impacts placental morphogenesis. Transgenic expression of human C19MC miRNAs impacts placental morphogenesis

Transgenic mice carrying the human C19MC locus expressing a family of primate-specific microRNAs (miRNAs) were generated. Similar to humans, the expression of C19MC miRNAs was mainly detected in mouse placentas. We use mouse gene expression microarrays to analyze the impact of the transgenic miRNAs on mouse placenta gene expression profiles. The transgenic mice in this study were bred to homozygocy and are referred to as dTG. Overall design: Total RNA were isolated from two placentas collected from a wild type pregnant mouse and six placentas collected from two transgenic females. Some embryos from dTG mice were substantially smaller in size although it did not reach statistical significance