Purification of LipBL.
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资源简介:
1: Incubation the E. coli/pFP crude extract in presence of sulfopropyl agarose. 2: Incubation the E. coli/pFP crude extract in presence of dextran-sulfate agarose and elution with 350 mM NaCl. 3: Incubation the E. coli/pFP crude extract in presence of IDA-Cu, elution with 100 mM imidazole and dialysis. 4: Incubation the E. coli/pFP crude extract in presence of octyl agarose, elution with 0.3% of Tritón X-100 and dialysis.
创建时间:
2011-08-10



